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Restriction Analysis
Input: a valid nucleotide sequence in *.prt files in database directory (help on how to import your sequence: please see "add_howtos.txt" for more information).
Results: base-count, restriction sites positions, melting point calculation for DNA, restriction map, plasmid/linear DNA drawing
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Protein Analysis
Input: a valid nucleotide sequence in *.prt files in database directory (help on how to import your sequence: please see "add_howtos.txt" for more information).
Results: translation, amino acid count,
disulfide bridges count, chemical properties of protein calculation, codon usage, prediction of
the secondary protein structure.
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Molecular Weight Calculator
Input: a valid nucleotide sequence in *.prt files in database directory (help on how to import your sequence: please see "add_howtos.txt" for more information).
Results: Calculation of the molecular weight for double-/single-strand DNA in g/mol
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Dotplot
Input: a valid nucleotide or amino acid sequence in the database directory (help on how to import your sequence: please see "add_howtos.txt" for more information).
Results: Dotplot graph with alignment data
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Open Reading Frame (ORF) Search
Input: a valid nucleotide sequence in *.prt files in database directory (help on how to import your sequence: please see "add_howtos.txt" for more information).
Results: positions of the ORFs
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Isoelectric Point (pI) Calculation
Input: a valid AMINO ACID sequence. If
the user has got only a DNA file, it is possible to create a corresponding
AA sequence using "Sequence Translator"-feature in "Extras"
Results: protein charge graph as a pH function
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Primer design
Input: 5'-3' primer sequence.
Results: melting point calculation, hybridisation, self-hybridisation, homology search
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Additional Programs (Plugins)
The program files can be found in the "PLUGINS" directory (*.DLL files). They will be automatically
recodnized by the main application. You can launch these programs from "Plugins" menu tab within the MB's interface
To download new plugins or update the installed versions please use automatic update function (you will have to
register the program, see here). Otherwise please check regulary the official homepage to make sure
not to miss some important updates.
If you want to develop your own plugin for MB please read this document.
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MALIGN.DLL
Description: Hierarchical Multiple Sequence Alignment
Input: a valid DNA/amino acid sequence
Output: multiple alignment, phylogenetic tree
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HELIX.DLL
Description: A basic alpha-helix analysis tool (draws spirals and calculates
the hydrophobicity graph)
Input: a valid amino acid sequence
Output: helix graph, hydrophobicity graph
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