Restriction Enzymes Positions Report:
Restriction map positions for 'pbr322.prt' File generated by MB DNA Analysis! On 14 08 05 12:20:34.421 ========================= Sequence: "pbr322.prt" Base count: Adenine: 984 (23%) Guanine: 1134 (26%) Thymine: 1034 (24%) Cytosine: 1210 (28%) Total: 4362 Approximate melting point (by GC percantage, double-strand DNA): Temperature Celsius: 60 ----- Promoter analysis: Sequence| Start| Transcription Start TATA-BOX| 2611| 2636| TATA-BOX| 4320| 4345| CAAT-BOX| 3256| 3331| Pribnow-Box| 10| 20| ----- Restriction sites search results: Search for cuts by 22 restriction enzymes Results for AfeI (agcgct) restriction site (positions): 232 494 775 1727 (Number of sites found: 4) Results for EcoRV (gatatc) restriction site (positions): 185 (Number of sites found: 1) Results for Eco52I (cggccg) restriction site (positions): 939 (Number of sites found: 1) Results for Eco47III (agcgct) restriction site (positions): 232 494 775 1727 (Number of sites found: 4) Results for Acc16I (tgcgca) restriction site (positions): 260 1356 1454 3586 (Number of sites found: 4) Results for XmaI (cccggg) restriction site (positions): (NO RESULTS) Results for XcmI (ccannnnnnnnntgg) restriction site (positions): (NO RESULTS) Results for XceI (rcatgy) restriction site (positions): (NO RESULTS) Results for XagI (cctnnnnnagg) restriction site (positions): 622 (Number of sites found: 1) Results for VspI (attaat) restriction site (positions): 3537 (Number of sites found: 1) Results for TspEI (aatt) restriction site (positions): 59 252 1320 1334 3234 3540 3795 4360 4361 4362 (Number of sites found: 10) Results for Tru9I (ttaa) restriction site (positions): 34 56 1720 1940 1972 2254 3179 3231 3236 3250 3303 3538 3577 3942 4314 (Number of sites found: 15) Results for EcoT22I (atgcat) restriction site (positions): (NO RESULTS) Results for EcoT14I (ccwwgg) restriction site (positions): 1369 (Number of sites found: 1) Results for EcoRII (ccwgg) restriction site (positions): 130 1059 1442 2500 2621 2634 (Number of sites found: 6) Results for EcoRI (gaattc) restriction site (positions): 4359 (Number of sites found: 1) Results for EcoO65I (ggtnacc) restriction site (positions): (NO RESULTS) Results for EcoO109I (rggnccy) restriction site (positions): (NO RESULTS) Results for EcoNI (cctnnnnnagg) restriction site (positions): 622 (Number of sites found: 1) Results for EcoICRI (gagctc) restriction site (positions): (NO RESULTS) Results for EcoHI (ccsgg) restriction site (positions): 170 534 1258 1484 1812 2118 2153 2852 3548 3899 (Number of sites found: 10) Results for Eco91I (ggtnacc) restriction site (positions): (NO RESULTS) - Unique restriction sites: - EcoRV Eco52I XagI VspI EcoT14I EcoRI EcoNI - Number of unique sites: 7 -Restriction Map:
Restriction map positions for 'pbr322.prt' Map created by MB DNA Analysis On 14 08 05 12:20:34.984 Enzymes cut after "^" Enzymes which cut more than 8 times are not displayed 1 ---------+---------+---------+---------+---------+---------+ 60 ttctcatgtttgacagcttatcatcgataagctttaatgcggtagtttatcacagttaaa 61 ---------+---------+---------+---------+---------+---------+ 120 ttgctaacgcagtcaggcaccgtgtatgaaatctaacaatgcgctcatcgtcatcctcgg 121 ---------+---------+---------+---------+---------+---------+ 180 caccgtcaccctggatgctgtaggcataggcttggttatgccggtactgccgggcctctt ^ EcoRII 181 ---------+---------+---------+---------+---------+---------+ 240 gcgggatatcgtccattccgacagcatcgccagtcactatggcgtgctgctagcgctata ^ ^ EcoRV AfeI Eco47III 241 ---------+---------+---------+---------+---------+---------+ 300 tgcgttgatgcaatttctatgcgcacccgttctcggagcactgtccgaccgctttggccg ^ Acc16I 301 ---------+---------+---------+---------+---------+---------+ 360 ccgcccagtcctgctcgcttcgctacttggagccactatcgactacgcgatcatggcgac 361 ---------+---------+---------+---------+---------+---------+ 420 cacacccgtcctgtggatcctctacgccggacgcatcgtggccggcatcaccggcgccac 421 ---------+---------+---------+---------+---------+---------+ 480 aggtgcggttgctggcgcctatatcgccgacatcaccgatggggaagatcgggctcgcca 481 ---------+---------+---------+---------+---------+---------+ 540 cttcgggctcatgagcgcttgtttcggcgtgggtatggtggcaggccccgtggccggggg ^ AfeI Eco47III 541 ---------+---------+---------+---------+---------+---------+ 600 actgttgggcgccatctccttgcatgcaccattccttgcggcggcggtgctcaacggcct 601 ---------+---------+---------+---------+---------+---------+ 660 caacctactactgggctgcttcctaatgcaggagtcgcataagggagagcgtcgaccgat ^ XagI EcoNI 661 ---------+---------+---------+---------+---------+---------+ 720 gcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgt 721 ---------+---------+---------+---------+---------+---------+ 780 cgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgct ^ AfeI Eco47III 781 ---------+---------+---------+---------+---------+---------+ 840 ctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgct 841 ---------+---------+---------+---------+---------+---------+ 900 tgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaa 901 ---------+---------+---------+---------+---------+---------+ 960 acgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgt ^ Eco52I 961 ---------+---------+---------+---------+---------+---------+ 1020 cttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttc 1021 ---------+---------+---------+---------+---------+---------+ 1080 cggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgacca ^ EcoRII 1081 ---------+---------+---------+---------+---------+---------+ 1140 tcagggacagcttcaaggatcgctcgcggctcttaccagcctaacttcgatcactggacc 1141 ---------+---------+---------+---------+---------+---------+ 1200 gctgatcgtcacggcgatttatgccgcctcggcgagcacatggaacgggttggcatggat 1201 ---------+---------+---------+---------+---------+---------+ 1260 tgtaggcgccgccctataccttgtctgcctccccgcgttgcgtcgcggtgcatggagccg 1261 ---------+---------+---------+---------+---------+---------+ 1320 ggccacctcgacctgaatggaagccggcggcacctcgctaacggattcaccactccaaga 1321 ---------+---------+---------+---------+---------+---------+ 1380 attggagccaatcaattcttgcggagaactgtgaatgcgcaaaccaacccttggcagaac ^ ^ Acc16I EcoT14I 1381 ---------+---------+---------+---------+---------+---------+ 1440 atatccatcgcgtccgccatctccagcagccgcacgcggcgcatctcgggcagcgttggg 1441 ---------+---------+---------+---------+---------+---------+ 1500 tcctggccacgggtgcgcatgatcgtgctcctgtcgttgaggacccggctaggctggcgg ^ ^ EcoRII Acc16I 1501 ---------+---------+---------+---------+---------+---------+ 1560 ggttgccttactggttagcagaatgaatcaccgatacgcgagcgaacgtgaagcgactgc 1561 ---------+---------+---------+---------+---------+---------+ 1620 tgctgcaaaacgtctgcgacctgagcaacaacatgaatggtcttcggtttccgtgtttcg 1621 ---------+---------+---------+---------+---------+---------+ 1680 taaagtctggaaacgcggaagtcagcgccctgcaccattatgttccggatctgcatcgca 1681 ---------+---------+---------+---------+---------+---------+ 1740 ggatgctgctggctaccctgtggaacacctacatctgtattaacgaagcgctggcattga ^ AfeI Eco47III 1741 ---------+---------+---------+---------+---------+---------+ 1800 ccctgagtgatttttctctggtcccgccgcatccataccgccagttgtttaccctcacaa 1801 ---------+---------+---------+---------+---------+---------+ 1860 cgttccagtaaccgggcatgttcatcatcagtaacccgtatcgtgagcatcctctctcgt 1861 ---------+---------+---------+---------+---------+---------+ 1920 ttcatcggtatcattacccccatgaacagaaatcccccttacacggaggcatcagtgacc 1921 ---------+---------+---------+---------+---------+---------+ 1980 aaacaggaaaaaaccgcccttaacatggcccgctttatcagaagccagacattaacgctt 1981 ---------+---------+---------+---------+---------+---------+ 2040 ctggagaaactcaacgagctggacgcggatgaacaggcagacatctgtgaatcgcttcac 2041 ---------+---------+---------+---------+---------+---------+ 2100 gaccacgctgatgagctttaccgcagctgcctcgcgcgtttcggtgatgacggtgaaaac 2101 ---------+---------+---------+---------+---------+---------+ 2160 ctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagc 2161 ---------+---------+---------+---------+---------+---------+ 2220 agacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggcgcagccatgacc 2221 ---------+---------+---------+---------+---------+---------+ 2280 cagtcacgtagcgatagcggagtgtatactggcttaactatgcggcatcagagcagattg 2281 ---------+---------+---------+---------+---------+---------+ 2340 tactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatacc 2341 ---------+---------+---------+---------+---------+---------+ 2400 gcatcaggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgc 2401 ---------+---------+---------+---------+---------+---------+ 2460 ggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggata 2461 ---------+---------+---------+---------+---------+---------+ 2520 acgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccg ^ EcoRII 2521 ---------+---------+---------+---------+---------+---------+ 2580 cgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgct 2581 ---------+---------+---------+---------+---------+---------+ 2640 caagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaa ^ ^ EcoRII EcoRII 2641 ---------+---------+---------+---------+---------+---------+ 2700 gctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttc 2701 ---------+---------+---------+---------+---------+---------+ 2760 tcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgt 2761 ---------+---------+---------+---------+---------+---------+ 2820 aggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcg 2821 ---------+---------+---------+---------+---------+---------+ 2880 ccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactgg 2881 ---------+---------+---------+---------+---------+---------+ 2940 cagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttct 2941 ---------+---------+---------+---------+---------+---------+ 3000 tgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgc 3001 ---------+---------+---------+---------+---------+---------+ 3060 tgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccg 3061 ---------+---------+---------+---------+---------+---------+ 3120 ctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctc 3121 ---------+---------+---------+---------+---------+---------+ 3180 aagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgtt 3181 ---------+---------+---------+---------+---------+---------+ 3240 aagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaa 3241 ---------+---------+---------+---------+---------+---------+ 3300 aatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaat 3301 ---------+---------+---------+---------+---------+---------+ 3360 gcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcct 3361 ---------+---------+---------+---------+---------+---------+ 3420 gactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctg 3421 ---------+---------+---------+---------+---------+---------+ 3480 caatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccag 3481 ---------+---------+---------+---------+---------+---------+ 3540 ccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctatta ^ VspI 3541 ---------+---------+---------+---------+---------+---------+ 3600 attgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttg ^ Acc16I 3601 ---------+---------+---------+---------+---------+---------+ 3660 ccattgctgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccg 3661 ---------+---------+---------+---------+---------+---------+ 3720 gttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagct 3721 ---------+---------+---------+---------+---------+---------+ 3780 ccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggtta 3781 ---------+---------+---------+---------+---------+---------+ 3840 tggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactg 3841 ---------+---------+---------+---------+---------+---------+ 3900 gtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcc 3901 ---------+---------+---------+---------+---------+---------+ 3960 cggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattg 3961 ---------+---------+---------+---------+---------+---------+ 4020 gaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcga 4021 ---------+---------+---------+---------+---------+---------+ 4080 tgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctg 4081 ---------+---------+---------+---------+---------+---------+ 4140 ggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaat 4141 ---------+---------+---------+---------+---------+---------+ 4200 gttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtc 4201 ---------+---------+---------+---------+---------+---------+ 4260 tcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgca 4261 ---------+---------+---------+---------+---------+---------+ 4320 catttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacct 4321 ---------+---------+---------+---------+---------+---------+ 4380 ataaaaataggcgtatcacgaggccctttcgtcttcaagaa ^ EcoRIPlasmid Map:
Restriction Enzymes are plotted on the map, as well as their cuts positions
Legend:
Sense ORFs are marked Green
AntiSense ORFs are marked Red
Frame #1
Minimal length of ORF: 200
Annotations to the map:
tet (Tc) (Gene) 86-1276 (Lime, Sense)
rop (Gene) 1915-2106 (Blue, Sense)
rep (pMB1) (Gene) 2519-3133 (Yellow, Antisense)
bla (Ap) (Gene) 3293-4153 (Black, Antisense)
ORF Report
Search ORF for: pbr322.prt Length of sequence: 4361 Sense ORFs found: ------------------- Frame: 2 86 .. 1276 Length: 1191 (396 amino acids) Frame: 2 158 .. 1276 Length: 1119 (372 amino acids) Frame: 2 248 .. 1276 Length: 1029 (342 amino acids) Frame: 1 259 .. 642 Length: 384 (127 amino acids) Frame: 2 353 .. 1276 Length: 924 (307 amino acids) Frame: 2 491 .. 1276 Length: 786 (261 amino acids) Frame: 2 515 .. 1276 Length: 762 (253 amino acids) Frame: 2 626 .. 1276 Length: 651 (216 amino acids) Frame: 2 659 .. 1276 Length: 618 (205 amino acids) Frame: 2 710 .. 1276 Length: 567 (188 amino acids) Frame: 2 731 .. 1276 Length: 546 (181 amino acids) Frame: 2 749 .. 1276 Length: 528 (175 amino acids) Frame: 2 824 .. 1276 Length: 453 (150 amino acids) Frame: 2 935 .. 1276 Length: 342 (113 amino acids) Frame: 2 989 .. 1276 Length: 288 (95 amino acids) Frame: 2 1004 .. 1276 Length: 273 (90 amino acids) Frame: 2 1034 .. 1276 Length: 243 (80 amino acids) Frame: 2 1052 .. 1276 Length: 225 (74 amino acids) Frame: 1 1180 .. 1518 Length: 339 (112 amino acids) Frame: 1 1195 .. 1518 Length: 324 (107 amino acids) Frame: 1 1252 .. 1518 Length: 267 (88 amino acids) Frame: 2 1523 .. 1723 Length: 201 (66 amino acids) Frame: 3 1593 .. 1811 Length: 219 (72 amino acids) Frame: 1 1882 .. 2106 Length: 225 (74 amino acids) Frame: 2 2009 .. 2218 Length: 210 (69 amino acids) Frame: 3 3423 .. 3689 Length: 267 (88 amino acids) Antisense ORFs found: ----------------------- Frame: -1 4153 .. 3293 Length: 861 (286 amino acids) Frame: -1 3958 .. 3293 Length: 666 (221 amino acids) Frame: -1 3955 .. 3293 Length: 663 (220 amino acids) Frame: -1 3811 .. 3293 Length: 519 (172 amino acids) Frame: -1 3775 .. 3293 Length: 483 (160 amino acids) Frame: -1 3697 .. 3293 Length: 405 (134 amino acids) Frame: -1 3616 .. 3293 Length: 324 (107 amino acids) Frame: -1 3604 .. 3293 Length: 312 (103 amino acids) Frame: -1 3529 .. 3293 Length: 237 (78 amino acids) Frame: -2 1883 .. 1515 Length: 369 (122 amino acids) Frame: -2 1874 .. 1515 Length: 360 (119 amino acids) Frame: -2 1865 .. 1515 Length: 351 (116 amino acids) Frame: -2 1850 .. 1515 Length: 336 (111 amino acids) Frame: -2 1772 .. 1515 Length: 258 (85 amino acids) Frame: -1 1081 .. 623 Length: 459 (152 amino acids) Frame: -3 789 .. 439 Length: 351 (116 amino acids) Frame: -3 750 .. 439 Length: 312 (103 amino acids) Frame: -3 711 .. 439 Length: 273 (90 amino acids) Frame: -1 565 .. 323 Length: 243 (80 amino acids)Copyright (c) MB DNA Analysis Visit http://www.molbiosoft.de for more information